Specimen entry

Accession number: ZFMK-TIS-8004932

Stable URL: https://id.zfmk.de/collection_ZFMK/8004932, other formats: rdf, json

Sample origin
Locality description:Nakerala Pass
Sampling plotEuropa
Coordinates (WGS 84): Lat: 42.37701 | Long: 43.03681 - Map
Collection date: 2018-7-18
Organism

Acalles

Identified by: Stüben, Peter

Identified at 2019-5-2

Curculionidae-8004932

Previous Identifications (expand +)


Order: Coleoptera

Family: Curculionidae

Sex: female

Number of individuals: 1


Material type: tissue sample

Storage location: ZFMK-Tissue-Bank_1210/46

Preparation method: 96% pure ethanol; +30°C for 1 month


Collection: GGBC-Coleoptera

Collection Contact: Björn Rulik

Accession number: ZFMK-TIS-8004932


Analyses: (expand +)


Analysis Barcode_Analyse161:

Analysis Description:Barcodes for ZFMK using Methods
Analysis date:2019-09-18 10:35:38
Analysis conducted by:GBOL-ZFMKLab

Analysis Result:

Methods:


Barcode

Gene locus: COI

project:
GBOL
sequence_length:
658
trace_count:
2

Consensus sequence:


Sequencing

Sequencing laboratory:BGI
Sequencing direction:forward
Sequence ID:248508
Primer name:LCO1490-JJ
pcr_primer_forward_name:
LCO1490-JJ
pcr_primer_forward_sequence:
CHACWAAYCATAAAGATATYGG
pcr_primer_reverse_name:
HCO2198-JJ
pcr_primer_reverse_sequence:
AWACTTCVGGRTGVCCAAARAATCA
sequencing_timestamp:
2019-06-17 00:00:00
trace_filename:
ZFMK-TIS-8004932__LCO1490-JJ__G02_1906090515G.ab1
trace_file_org_length:
262738
trace_file_org_md5:
cff901c3fe9dcfb7f9ad4b71dea18289
trace_file_enc_length:
350320
trace_file_format:
ABI
trace_id:
214565
sequencing_primer_sequence:
CHACWAAYCATAAAGATATYGG
marker_code:
COI


Sequencing

Sequencing laboratory:BGI
Sequencing direction:reverse
Sequence ID:248413
Primer name:HCO2198-JJ
pcr_primer_forward_name:
LCO1490-JJ
pcr_primer_forward_sequence:
CHACWAAYCATAAAGATATYGG
pcr_primer_reverse_name:
HCO2198-JJ
pcr_primer_reverse_sequence:
AWACTTCVGGRTGVCCAAARAATCA
sequencing_timestamp:
2019-06-17 00:00:00
trace_filename:
ZFMK-TIS-8004932__HCO2198-JJ__G02_1906090515G.ab1
trace_file_org_length:
254297
trace_file_org_md5:
1c75cce7533c84ae7b544d2e6d8114de
trace_file_enc_length:
339064
trace_file_format:
ABI
trace_id:
214470
sequencing_primer_sequence:
AWACTTCVGGRTGVCCAAARAATCA
marker_code:
COI



Analysis Barcode_PCR304:

Analysis Description:PCRs used to sequence Barcodes.
Analysis date:2018-10-23 00:00:00
Analysis Result: LCO1490-JJ/HCO2198-JJ: suspect

Methods:


PCR

pcr_primer_fwd_name:
LCO1490-JJ
pcr_primer_fwd_sequence:
CHACWAAYCATAAAGATATYGG
lims_plate_name:
GBOL_PCR_1069
date:
2018-10-23 00:00:00
status:
suspect
lims_workflow_id:
115498
pcr_primer_rev_name:
HCO2198-JJ
pcr_primer_rev_sequence:
AWACTTCVGGRTGVCCAAARAATCA


entry number 1

https://media.leibniz-lib.de/eaurls/image/152173/full/small/0/ZFMK_8004932.png

Licensed with: by Zoological Research Museum Alexander Koenig