Specimen entry

Accession number: ZFMK-TIS-2567958

Stable URL: https://id.zfmk.de/collection_ZFMK/2567958, other formats: rdf, json

Sample origin

Locality description:	Ljubljana
Sampling plot	Europa
Coordinates (WGS 84):	Lat: 46.04550 \| Long: 14.52234 - Map
Collection date:	2015-12-19

Organism

Acanthoscelides obtectus (Say, 1831)

(http://services.snsb.info/DTNtaxonlists/rest/v0.1/names/TaxonNames_Insecta/5007332)

Identified by: Köhler, Frank

Identified at 2016-11-30

Order: Coleoptera

Family: Bruchidae

Life stage: adult

Number of individuals: 1

Material type: tissue sample

Storage location: ZFMK-Tissue-Bank_744/51

Preparation method: Ethanol 96%

Collection: GBOL-Coleoptera

Collection Contact: Björn Rulik

Accession number: ZFMK-TIS-2567958

Analysis Barcode_Analyse161:

Analysis Description:	Barcodes for ZFMK using Methods
Analysis date:	2017-03-31 16:16:39
Analysis conducted by:	GBOL-ZFMKLab
Analysis Result: AACTTTATACTTTCTATTTGGAGCTTGAGCTGGAATAGCTGGAACTTCACTTAGAATCTTAATTCGAGCCGAATTAGGAAACCCGGGCTCTCTAATTGGAAATGACCAAATTTATAATGTAATTGTTACTGCACACGCATTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTACCTTTAATACTGGGGGCACCAGATATGGCCTTTCCCCGAATAAATAATATAAGATTTTGACTTTTACCCCCATCATTAAGATTCCTCATATTAAGAAGTTTAGTAGAAAGAGGAGCCGGGACAGGTTGAACTGTCTATCCCCCCTTAGCAAGTAATATTGCTCATAGAGGATCTTCCGTTGATTTAGCAATTTTTAGACTACATTTAGCCGGAATTTCTTCTATTTTAGGAGCAGTTAATTTTATTACAACTATTATAAATATGCGACCAAATGGTATAAGGATAGATCGAATGCCTTTATTTTCTTGAGCAGTTATAATTACAGCTATTCTTTTGCTTCTTTCTCTACCAGTACTAGCAGGGGCAATTACTATACTTCTTACTGATCGAAACTTAAATACATCCTTTTTTGACCCTGCGGGAGGGGGGGATCCTATTTTATACCAACACTTATTT

Methods:

Barcode

Gene locus: COI

project:	GBOL
sequence_length:	658
trace_count:	2

Consensus sequence:

AACTTTATACTTTCTATTTGGAGCTTGAGCTGGAATAGCTGGAACTTCACTTAGAATCTTAATTCGAGCCGAATTAGGAAACCCGGGCTCTCTAATTGGAAATGACCAAATTTATAATGTAATTGTTACTGCACACGCATTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTACCTTTAATACTGGGGGCACCAGATATGGCCTTTCCCCGAATAAATAATATAAGATTTTGACTTTTACCCCCATCATTAAGATTCCTCATATTAAGAAGTTTAGTAGAAAGAGGAGCCGGGACAGGTTGAACTGTCTATCCCCCCTTAGCAAGTAATATTGCTCATAGAGGATCTTCCGTTGATTTAGCAATTTTTAGACTACATTTAGCCGGAATTTCTTCTATTTTAGGAGCAGTTAATTTTATTACAACTATTATAAATATGCGACCAAATGGTATAAGGATAGATCGAATGCCTTTATTTTCTTGAGCAGTTATAATTACAGCTATTCTTTTGCTTCTTTCTCTACCAGTACTAGCAGGGGCAATTACTATACTTCTTACTGATCGAAACTTAAATACATCCTTTTTTGACCCTGCGGGAGGGGGGGATCCTATTTTATACCAACACTTATTT

Sequencing

Sequencing laboratory:	BGI
Sequencing direction:	forward
Sequence ID:	183730
Primer name:	LCO1490-JJ
pcr_primer_forward_name:	LCO1490-JJ
pcr_primer_forward_sequence:	CHACWAAYCATAAAGATATYGG
pcr_primer_reverse_name:	HCO2198-JJ
pcr_primer_reverse_sequence:	AWACTTCVGGRTGVCCAAARAATCA
sequencing_timestamp:	2017-02-07 00:00:00
trace_filename:	ZFMK-TIS-2567958__LCO1490-JJ_A63544_D01_1703401504G.ab1
trace_file_org_length:	263600
trace_file_org_md5:	d81c2c6cc5336a282b7767944ea7e433
trace_file_enc_length:	351468
trace_file_format:	ABI
trace_id:	157224
sequencing_primer_sequence:	CHACWAAYCATAAAGATATYGG
marker_code:	COI

Sequencing

Sequencing laboratory:	BGI
Sequencing direction:	reverse
Sequence ID:	183634
Primer name:	HCO2198-JJ
pcr_primer_forward_name:	LCO1490-JJ
pcr_primer_forward_sequence:	CHACWAAYCATAAAGATATYGG
pcr_primer_reverse_name:	HCO2198-JJ
pcr_primer_reverse_sequence:	AWACTTCVGGRTGVCCAAARAATCA
sequencing_timestamp:	2017-02-07 00:00:00
trace_filename:	ZFMK-TIS-2567958__HCO2198-JJ_A63544_D01_1703401504G.ab1
trace_file_org_length:	263516
trace_file_org_md5:	1f7f0fd41c44869275b8b0cc7c7787ee
trace_file_enc_length:	351356
trace_file_format:	ABI
trace_id:	157129
sequencing_primer_sequence:	AWACTTCVGGRTGVCCAAARAATCA
marker_code:	COI

entry number 1

https://media.leibniz-lib.de/eaurls/image/67806/full/small/0/ZFMK_2567958.png

Licensed with: by Zoological Research Museum Alexander Koenig