Specimen entry

Accession number: ZFMK-TIS-2512825

Stable URL: https://id.zfmk.de/collection_ZFMK/2512825, other formats: rdf, json

Sample origin

Locality description:	Borkum, Greune Stee
Sampling plot	Niedersachsen
Coordinates (WGS 84):	Lat: 53.57472 \| Long: 6.707324 - Map
Collection date:	2013-10-12

Organism

Acilius sulcatus (L., 1758)

(http://services.snsb.info/DTNtaxonlists/rest/v0.1/names/TaxonNames_Insecta/5005467)

Identified by: Köhler, Jonas

Identified at 2013-12-5

Order: Coleoptera

Family: Dytiscidae

Life stage: adult

Number of individuals: 1

Material type: tissue sample

Storage location: ZFMK-Tissue-Bank_296/50

Preparation method: 96% pure ethanol

Collection: GBOL-Coleoptera

Collection Contact: Björn Rulik

Accession number: ZFMK-TIS-2512825

Analysis Barcode_Analyse161:

Analysis Description:	Barcodes for ZFMK using Methods
Analysis date:	2016-04-11 11:31:23
Analysis conducted by:	GBOL-ZFMKLab
Analysis Result: AACTTTATATTTTATCTTTGGGCCATGATCAGGAATAGTAGGAACATCTTTAAGAATATTAATTCGAGCAGAACTAGGAAACCCGGGCTCATTAATTGGAGATGATCAGATCTATAACGTTATTGTAACTGCTCATGCATTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGAGCTCCTGATATAGCTTTCCCACGTATAAATAATATAAGATTTTGATTATTACCCCCTTCTTTAACTTTATTATTAATAAGAAGAATAGTAGAAAATGGAGCAGGGACAGGATGAACAGTTTATCCACCCCTTTCAGCAGGAATTGCTCACGGGGGAGCTTCAGTAGATTTAGCTATTTTTAGATTACATTTAGCTGGAATTTCTTCAATTTTAGGGGCTGTAAATTTTATTACAACTATTATTAATATACGATCAGTAGGAATAACATTTGATCGAATACCTTTATTTGTTTGATCAGTGGGAATTACTGCTTTATTATTATTATTATCACTACCTGTATTAGCGGGAGCTATTACCATATTATTAACTGACCGAAATTTAAATACTTCTTTTTTTGATCCTGCAGGAGGAGGGGACCCAATTCTTTACCAGCATTTATTT

Methods:

Barcode

Gene locus: COI

project:	GBOL
sequence_length:	658
trace_count:	2

Consensus sequence:

AACTTTATATTTTATCTTTGGGCCATGATCAGGAATAGTAGGAACATCTTTAAGAATATTAATTCGAGCAGAACTAGGAAACCCGGGCTCATTAATTGGAGATGATCAGATCTATAACGTTATTGTAACTGCTCATGCATTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGAGCTCCTGATATAGCTTTCCCACGTATAAATAATATAAGATTTTGATTATTACCCCCTTCTTTAACTTTATTATTAATAAGAAGAATAGTAGAAAATGGAGCAGGGACAGGATGAACAGTTTATCCACCCCTTTCAGCAGGAATTGCTCACGGGGGAGCTTCAGTAGATTTAGCTATTTTTAGATTACATTTAGCTGGAATTTCTTCAATTTTAGGGGCTGTAAATTTTATTACAACTATTATTAATATACGATCAGTAGGAATAACATTTGATCGAATACCTTTATTTGTTTGATCAGTGGGAATTACTGCTTTATTATTATTATTATCACTACCTGTATTAGCGGGAGCTATTACCATATTATTAACTGACCGAAATTTAAATACTTCTTTTTTTGATCCTGCAGGAGGAGGGGACCCAATTCTTTACCAGCATTTATTT

Sequencing

Sequencing laboratory:	BGI
Sequencing direction:	forward
Sequence ID:	144310
Primer name:	LCO1490-JJ2
pcr_primer_forward_name:	LCO1490-JJ2
pcr_primer_forward_sequence:	CHACWAAYCAYAARGAYATYGG
pcr_primer_reverse_name:	HCO2198-JJ2
pcr_primer_reverse_sequence:	ANACTTCNGGRTGNCCAAARAATCA
sequencing_timestamp:	2016-02-29 00:00:00
trace_filename:	ZFMK-TIS-2512825__LCO1490-JJ2_A63544_C06_1603100143G.ab1
trace_file_org_length:	261291
trace_file_org_md5:	311cf51e3283c4ee3b8af6b9adb6212a
trace_file_enc_length:	348388
trace_file_format:	ABI
trace_id:	124377
sequencing_primer_sequence:	CHACWAAYCAYAARGAYATYGG
marker_code:	COI

Sequencing

Sequencing laboratory:	BGI
Sequencing direction:	reverse
Sequence ID:	144406
Primer name:	HCO2198-JJ2
pcr_primer_forward_name:	LCO1490-JJ2
pcr_primer_forward_sequence:	CHACWAAYCAYAARGAYATYGG
pcr_primer_reverse_name:	HCO2198-JJ2
pcr_primer_reverse_sequence:	ANACTTCNGGRTGNCCAAARAATCA
sequencing_timestamp:	2016-02-29 00:00:00
trace_filename:	ZFMK-TIS-2512825__HCO2198-JJ2_A63544_C06_1603100143G.ab1
trace_file_org_length:	261834
trace_file_org_md5:	8967a055427f2d98150add6baed02b22
trace_file_enc_length:	349112
trace_file_format:	ABI
trace_id:	124472
sequencing_primer_sequence:	ANACTTCNGGRTGNCCAAARAATCA
marker_code:	COI

entry number 1

https://media.leibniz-lib.de/eaurls/image/66695/full/small/0/ZFMK_2512825.png

Licensed with: by Zoological Research Museum Alexander Koenig